A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
Electron. j. biotechnol
; 14(1): 10-11, Jan. 2011. ilus, tab
Article
in En
| LILACS
| ID: lil-591928
Responsible library:
CL1.1
ABSTRACT
A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
DNA
/
Corchorus
Language:
En
Journal:
Electron. j. biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
India
Country of publication:
Chile