Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Biocell
; 34(1): 1-6, Apr. 2010. tab, ilus
Article
in En
| LILACS
| ID: lil-595044
Responsible library:
AR40.1
ABSTRACT
Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 microM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 microM elicit or, sampling at 10 days.
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Paclitaxel
/
Taxus
/
Cyclopentanes
/
Cell Proliferation
/
Alkaloids
/
Acetates
Language:
En
Journal:
Biocell
Journal subject:
Clulas
Year:
2010
Document type:
Article
Affiliation country:
Mexico
Country of publication:
Argentina