Assessment of Leishmania major and Leishmania braziliensis promastigote viability after photodynamic treatment with aluminum phthalocyanine tetrasulfonate (AlPcS4)
J. venom. anim. toxins incl. trop. dis
; 17(3): 300-307, 2011. graf, tab
Article
in En
| LILACS
| ID: lil-597229
Responsible library:
BR33.1
ABSTRACT
Cutaneous leishmaniasis is an infectious disease caused by protozoans of the genus Leishmania, which is transmitted through the bite of hematophagous insects of the genus Lutzomyia. This study aimed at testing in vitro the phototoxic effect of aluminum phthalocyanine tetrasulfonate (AlPcS4) on the viability of Leishmania major and Leishmania braziliensis. Stationary phase promastigote forms were treated with AlPcS4 at 1.0 µM and 10.0 µM and incubated for one hour. Then 659 nm laser was applied at 5 and 10 J/cm². Parasite viability was determined by differential count using the trypan blue dye exclusion method and by monitoring growth curves for nine days. Trypan blue exclusion assay showed a significant reduction of viable parasites compared to controls, L. major seemed more sensitive to the toxic effects of AlPcS4 in the dark. The most effective photodynamic therapy (PDT) was obtained with AlPcS4 at 10.0 µM and 10 J/cm² whereas L. braziliensis showed the highest mortality rate after treatment.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Photochemotherapy
/
Leishmania braziliensis
/
Leishmaniasis, Cutaneous
/
Photosensitizing Agents
/
Leishmania major
/
Antiparasitic Agents
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
TOXICOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Brazil