Survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction
Mem. Inst. Oswaldo Cruz
; 106(6): 763-768, Sept. 2011. tab
Article
in English
| LILACS
| ID: lil-602063
Responsible library:
BR1.1
ABSTRACT
This work reports a survey of Leptospira spp in pampas deer (Ozotoceros bezoarticus) in the Pantanal wetlands of the state of Mato Grosso do Sul, Brazil by serology and polymerase chain reaction (PCR). Seventy pampas deer were captured in the dry season and surveyed using PCR, microscopic agglutination test (MAT) (n = 51) and by both techniques (n = 47). PCR detected infections in two pampas deer and MAT detected infections in three. Through sequencing and phylogenetic analyses, the PCR-amplified fragment detected in deer was identified as Leptospira interrogans. Serovars Pomona and Butembo were detected using MAT and the highest titre was 200 for serovar Pomona. Epidemiological aspects of the findings are discussed.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
Deer
/
Leptospira interrogans
/
Leptospirosis
/
Antibodies, Bacterial
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Gado de Corte/BR
/
Embrapa Pantanal/BR
/
Independent Consultant on Veterinary/BR
/
Universidade Federal do Mato Grosso do Sul/BR
/
Universidade de Brasília/BR
/
Universidade de São Paulo/BR