Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
Genet. mol. biol
; Genet. mol. biol;35(1): 122-125, 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-616989
Responsible library:
BR1.1
ABSTRACT
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.
Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Pichia
/
Candida
/
DNA, Fungal
Type of study:
Diagnostic_studies
Language:
En
Journal:
Genet. mol. biol
/
Genet. mol. biol. (Online)
/
Genetics and molecular biology (Impresso)
Journal subject:
GENETICA
Year:
2012
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil