The effects of carvedilol on ischemia-reperfusion injury in the rat testis
Int. braz. j. urol
; 40(1): 109-117, Jan-Feb/2014. tab, graf
Article
in En
| LILACS
| ID: lil-704178
Responsible library:
BR1.1
ABSTRACT
Objective:
To analyze the oxidative damage and histopathological alterations caused by ischemia-reperfusion (I/R) injury and ameliorative effects of carvedilol (CVD) in the rat testis. Materials andMethods:
Twenty-one male rats were randomized into 3 groups as follows Group I (n = 7); control (sham) group, Group II (n = 7); I/R group, in which I/R injury was performed by torsing the left testis 720º clockwise for 2 hours and detorsing for 2 hours. Group III (n = 7); CVD treatment group; in addition to I/R process, one-dose of CVD was administered (2mg/kg, i.p) 30 min. before detorsion. Levels of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and levels of malondialdehyde (MDA) and protein carbonyl (PC) were determined in testicular tissues and serum of rats. Testicular tissues were also examined histopathologically and Johnsen scores were determined.Results:
Activities of SOD and GSH-Px in serum and testicular tissues were increased by I/R, but administration of CVD decreased these levels (p < 0.001 and p = 0.001). Significantly increased MDA levels in serum and testicular tissues were decreased by CVD treatment (p < 0.001 and p = 0.001). Concerning PC levels in serum and testicular tissues, there was no statistically significant difference between the groups (p = 0.989 and p = 0.428). There was not a statistically significant difference in terms of mean Johnsen scores between the groups (p = 0.161).Conclusions:
Administration of CVD decreased oxidative damage biochemically in the rat testis caused by I/R injury, but histopathologically no change was observed between all of the groups. .Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Propanolamines
/
Testis
/
Vasodilator Agents
/
Carbazoles
/
Reperfusion Injury
/
Oxidative Stress
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Int. braz. j. urol
Journal subject:
UROLOGIA
Year:
2014
Document type:
Article
Affiliation country:
Turkey
Country of publication:
Brazil