Biodegradation of [D-Leu1] microcystin-LR by a bacterium isolated from sediment of Patos Lagoon estuary, Brazil
J. venom. anim. toxins incl. trop. dis
; 21: 1-8, 31/03/2015. tab, graf
Article
in En
| LILACS
| ID: lil-741604
Responsible library:
BR33.1
ABSTRACT
Background:
Toxic cyanobacterial blooms are recurrent in Patos Lagoon, in southern Brazil. Among cyanotoxins, [D-Leu1] microcystin-LR is the predominant variant whose natural cycle involves water and sediment compartments. This study aimed to identify and isolate from sediment a bacterial strain capable of growing on [D-Leu1] microcystin-LR. Sediment and water samples were collected at two distinct aquatic spots close to the Oceanographic Museum (P1), in Rio Grande City, and on São Lourenço Beach (P2), in São Lourenço do Sul City, southern Brazil.Methods:
[D-Leu1] microcystin-LR was isolated and purified from batch cultures of Microcystis aeruginosastrain RST9501. Samples of water and sediment from Rio Grande and São Lourenço do Sul were collected. Bacteria from the samples were allowed to grow in flasks containing solely [D-Leu1] microcystin-LR. This strain named DMSX was isolated on agar MSM with 8 g L−1 glucose and further purified on a cyanotoxin basis growth. Microcystin concentration was obtained by using the ELISA immunoassay for microcystins whereas bacterial count was performed by epifluorescence microscopy. The genus Pseudomonas was identified by DNA techniques. Results; Although several bacterial strains were isolated from the samples, only one, DMXS, was capable of growing on [D-Leu1] microcystin-LR. The phylogenetic analysis of the 16S rRNA gene from DMXS strain classified the organism as Pseudomonas aeruginosa. DMXS strain incubated with [D-Leu1] microcystin-LR lowered the amount of toxin from 1 μg.L−1 to < 0.05 μg.L−1. Besides, an increase in the bacterial countfrom 71 × 105 bacteria.mL−1 to 117 × 105 bacteria.mL−1was observed along the incubation.Conclusions:
The use of bacteria isolated from sediment for technological applications to remove toxic compounds is viable. Studies have shown that sediment plays an important role as ...Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Biodegradation, Environmental
/
Water
/
Sediments
/
Cyanobacteria
/
Estuaries
/
Microcystins
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
TOXICOLOGIA
Year:
2015
Document type:
Article
Affiliation country:
Brazil
Country of publication:
Brazil