A modified method by differential adhesion for enrichment of bladder cancer stem cells
Int. braz. j. urol
; 42(4): 817-824, July-Aug. 2016. tab, graf
Article
in En
| LILACS
| ID: lil-794669
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Purpose:
In a previous study the vaccine was effective against bladder cancer in a mouse model. However, a small portion of tumors regrew because the vaccine could not eliminate bladder cancer stem cells (CSCs). In this study, we showed a modified method for the isolation of bladder CSCs using a combination of differential adhesion method and serum-free culture medium (SFM) method. Materials andMethods:
Trypsin-resistant cells and trypsin-sensitive cells were isolated from MB49, EJ and 5637 cells by a combination of differential adhesion method and SFM method. The CSCs characterizations of trypsin-resistant cells were verified by the flow cytometry, the western blotting, the quantitative polymerase chain reaction, the resistance to chemotherapy assay, the transwell assay, and the tumor xenograft formation assay.Results:
Trypsin-resistant cells were isolated and identified in CSCs characters, with high expression of CSCs markers, higher resistance to chemotherapy, greater migration in vitro, and stronger tumorigenicity in vivo.Conclusion:
Trypsin-resistant cells displayed specific CSCs properties. Our study showed trypsin-resistant cells were isolated successfully with a modified method using a combination of differential adhesion method and SFM method.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Neoplastic Stem Cells
/
Urinary Bladder Neoplasms
/
Trypsin
/
Cell Adhesion
/
Cell Separation
/
Cell Culture Techniques
Limits:
Animals
Language:
En
Journal:
Int. braz. j. urol
Journal subject:
UROLOGIA
Year:
2016
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil