Purification and characterization of a metalloprotease from Chryseobacterium indologenes Ix9a and determination of the amino acid specificity with electrospray mass spectrometry.

Venter, H; Osthoff, G; Litthauer, D

Venter, H; Osthoff, G; Litthauer, D.

Affiliation

Venter H; Department of Food Science, University of the Orange Free State, Bloemfontein, South Africa.

Protein Expr Purif ; 15(3): 282-95, 1999 Apr.

Article in En | MEDLINE | ID: mdl-10092488

Subject(s)

Flavobacterium/enzymology; Metalloendopeptidases/isolation & purification; Metalloendopeptidases/metabolism; Amino Acid Sequence; Amino Acids; Apoenzymes/metabolism; Calcium/analysis; Cations, Divalent/pharmacology; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Metalloendopeptidases/chemistry; Molecular Sequence Data; Molecular Weight; Peptide Fragments/chemistry; Protease Inhibitors/pharmacology; Sequence Alignment; Sequence Homology, Amino Acid; Spectrometry, Mass, Secondary Ion; Substrate Specificity; Thermodynamics; Zinc/analysis

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Collection: 01-internacional Database: MEDLINE Main subject: Metalloendopeptidases / Flavobacterium Type of study: Prognostic_studies Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 1999 Document type: Article Affiliation country: South Africa Country of publication: United States

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