The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae.
RNA
; 5(3): 360-8, 1999 Mar.
Article
in En
| MEDLINE
| ID: mdl-10094305
We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1 p is important in the optimization of splicing.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
RNA Splicing
/
RNA-Binding Proteins
/
Saccharomyces cerevisiae Proteins
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
RNA
Journal subject:
BIOLOGIA MOLECULAR
Year:
1999
Document type:
Article
Affiliation country:
United kingdom
Country of publication:
United States