Your browser doesn't support javascript.
loading
Determination of the histamine metabolite tele-methylimidazoleacetic acid and of creatinine in urine by the same HPLC system.
Granerus, G; Lönnqvist, B; Wass, U.
Affiliation
  • Granerus G; Department of Medicine and Care, University Hospital, Linköping, Sweden. goran.granerus@nuk.us.lio.se
Inflamm Res ; 48(2): 75-80, 1999 Feb.
Article in En | MEDLINE | ID: mdl-10202992
ABSTRACT
OBJECTIVE AND

DESIGN:

To develop an HPLC method with UV detection for determination of tele-methylimidazoleacetic acid (t-MelmAA) in urine of man or animals, which is reliable, simple and less expensive than existing GC/MS techniques.

METHOD:

The elaborated procedure enables separation of t-MelmAA from its pros-isomer (p-MelmAA) as well as from imidazoleacetic acid. As an internal standard tele-ethylimidazoleacetic acid (t-EtlmAA) is used. The acids, after being converted to their stable isopropylesters, are extracted at pH 6.0-6.5. The further separation prior to HPLC utilises a small cellulose phosphate column. The HPLC system is isocratic with a C18 column and mobile phase consisting of an aqueous solution of SDS at pH 3.5 mixed with acetonitrile (6535). An advantage of this system is that it can be used to determine the urine creatinine concentration to express excreted t-MelmAA in mmol/mol creatinine. RESULTS AND

CONCLUSIONS:

The method applicability is demonstrated in repeated studies in mastocytosis patients. The broad range of excretion values, from normal up to a high level (0.9 to 30 mmol/mol creatinine), indicates that it can be satisfactorily used for evaluation of histamine turnover under various clinical conditions. The method appears to be a good alternative to GC/MS based ones.
Subject(s)
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Creatinine / Imidazoles Limits: Adult / Humans Language: En Journal: Inflamm Res Journal subject: ALERGIA E IMUNOLOGIA / PATOLOGIA Year: 1999 Document type: Article Affiliation country: Sweden
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Creatinine / Imidazoles Limits: Adult / Humans Language: En Journal: Inflamm Res Journal subject: ALERGIA E IMUNOLOGIA / PATOLOGIA Year: 1999 Document type: Article Affiliation country: Sweden