Clusterin (SGP-2) gene expression is cell cycle dependent in normal human dermal fibroblasts.
FEBS Lett
; 448(2-3): 297-300, 1999 Apr 09.
Article
in En
| MEDLINE
| ID: mdl-10218496
ABSTRACT
In confluent human dermal fibroblasts brought to quiescence (G0) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G2/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G0, in parallel with h-gas1 mRNA. At 6 h (roughly G1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glycoproteins
/
Cell Cycle
/
Molecular Chaperones
/
Saccharomyces cerevisiae Proteins
/
Fibroblasts
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
1999
Document type:
Article
Affiliation country:
Italy
Country of publication:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM