Exogenous IL-10 and IL-4 down-regulate IL-6 production by SLE-derived PBMC.
Clin Immunol
; 91(1): 6-16, 1999 Apr.
Article
in En
| MEDLINE
| ID: mdl-10219249
ABSTRACT
The elevated expression of IL-6 and IL-10 may have an important role in SLE pathogenesis. IL-6 production by normal monocytes can be inhibited by IL-10, and it has been suggested that SLE monocytes are refractory to this negative signal. To examine this possibility, the effects of regulatory factors on IL-6 expression by SLE PBMC (N = 51) were compared to effects on control PBMC (N = 21). We found that (1) exogenous rIL-10 and rIL-4 mediated reduction of constitutive and lectin-induced IL-6 in monocytes of SLE patients as effectively as that of controls; (2) IL-6 mRNA decay was significantly delayed in SLE with active disease (P < 0.001); (3) adding rIL-10 or neutralizing endogenous IL-1 beta and TNF-alpha down-regulated IL-6 mainly by destabilizing IL-6 transcripts, whereas exogenous IL-4 and TGF beta 1 down-regulated IL-6 transcriptionally; (4) time kinetics and levels of IL-10 were lower than those of IL-6 and IL-1 beta. Thus, contrary to a previous report, IL-6 production by SLE PBMC responds normally to regulatory signals, and the IL-6 overexpression in SLE may be due, at least in part, to the kinetics and availability of regulatory cytokines.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Interleukin-4
/
Interleukin-6
/
Interleukin-10
/
Lupus Erythematosus, Systemic
Type of study:
Observational_studies
Limits:
Adult
/
Female
/
Humans
/
Middle aged
Language:
En
Journal:
Clin Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
1999
Document type:
Article
Affiliation country:
United States