Reed-Sternberg cell genome expression supports a B-cell lineage.
Blood
; 94(2): 411-6, 1999 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-10397707
ABSTRACT
The malignant Reed-Sternberg cell of Hodgkin's disease, first described a century ago, has resisted in-depth analysis due to its extreme rarity in lymphomatous tissue. To directly study its genome-wide gene expression, approximately 11,000,000 bases (27,518 cDNA sequences) of expressed gene sequence was determined from living single Reed-Sternberg cells, Hodgkin's tissue, and cell lines. This approach increased the number of genes known to be expressed in Hodgkin's disease by 20-fold to 2,666 named genes. The data here indicate that Reed-Sternberg cells from both nodular sclerosing and lymphocyte predominant Hodgkin's disease were derived from an unusual B-cell lineage based on a comparison of their gene expression to approximately 40,000,000 bases (10(5) sequences) of expressed gene sequence from germinal center B cells (GCB) and dendritic cells. The data set of expressed genes, reported here and on the World Wide Web, forms a basis to understand the genes responsible for Hodgkin's disease and develop novel diagnostic markers and therapies. This study of the rare Reed-Sternberg cell, concealed in its heterogenous cellular context, also provides a formidable test case to advance the limit of analysis of differential gene expression to the single disease cell.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hodgkin Disease
/
B-Lymphocytes
/
Gene Expression Regulation, Neoplastic
/
Reed-Sternberg Cells
/
Neoplasm Proteins
Limits:
Humans
Language:
En
Journal:
Blood
Year:
1999
Document type:
Article
Affiliation country:
United States