Stable long-term germ-line transmission of transgene integration sites in mice.
Transgenic Res
; 8(1): 1-8, 1999 Feb.
Article
in En
| MEDLINE
| ID: mdl-10399363
ABSTRACT
Transgenic mice provide a valuable tool in all fields of basic and applied biological and medical research. In this study, we describe the fate of integrated transgenes in the mammalian host genome over a large number of generations. The stability of the germ-line transmission of integrated tyrosinase transgene copies was monitored up to generation F20 in a large number of individuals from seven transgenic mouse lines. Phenotypic and molecular genetic analysis of the offspring both within the different lines and in cross-breeding experiments revealed the high stability of the transgene integration sites in mice. Only very few individuals were affected by a transgene copy loss. These results indicate that, once homozygous transgenic lines are established, breeding programs can be continued to a high number of generations without further stringent molecular genetic analysis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mice, Transgenic
/
Transgenes
/
Germ Cells
Limits:
Animals
Language:
En
Journal:
Transgenic Res
Journal subject:
BIOLOGIA MOLECULAR
Year:
1999
Document type:
Article
Affiliation country:
Austria