Detection of dendritic cells in the non-obese diabetic (NOD) mouse islet pancreas infiltrate is correlated with Th2-cytokine production.
J Cell Biochem
; 74(3): 447-57, 1999 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-10412045
ABSTRACT
We investigate the role played by dendritic cells (DCs) in the non-obese diabetic (NOD) mouse pancreas. The early peri-islet, nondestructive infiltration phase, and intra-islet, destructive infiltration phase, which immediately precedes overt diabetes, are studied. Results show that infiltrating cells are Ia-b, ICAM-1, and, mainly, MIDC-8 immunoreactive (ir). These data from silica-treated animals and ultrastructural observations strongly support the hypothesis that DCs are both Ia-b-ir and ICAM-1-ir and that they exert a pivotal role during the period of early infiltration. This is a novel finding for NOD mice and increases the interest for this protective cell type during the rather complex islet infiltration process. Moreover, the cytokine profile demonstrates that Th2 protective cytokines are specific for peri-islet infiltrate. Disappearance of DCs from the infiltrate is concomitant with both the formation of intra-islet infiltration and the increase in proinflammatory Th1 cytokine levels. This further supports the hypothesis that DCs may exert a protective role against diabetes development.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dendritic Cells
/
Cytokines
/
Islets of Langerhans
/
Th2 Cells
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Cell Biochem
Year:
1999
Document type:
Article
Affiliation country:
Italy