An adenovirus-Epstein-Barr virus hybrid vector that stably transforms cultured cells with high efficiency.
J Virol
; 73(9): 7582-9, 1999 Sep.
Article
in En
| MEDLINE
| ID: mdl-10438848
ABSTRACT
EBV episomes are nuclear plasmids that are stably maintained through multiple cell divisions in primate and canine cells (J. L. Yates, N. Warren, and B. Sugden, Nature 313812-815, 1985). In this report, we describe the construction and characterization of an E1-deleted recombinant adenovirus vector system that delivers an EBV episome to infected cells. This adenovirus-EBV hybrid vector system utilizes Cre-mediated, site-specific recombination to excise an EBV episome from a target recombinant adenovirus genome. We demonstrate that this vector system efficiently delivers the EBV episome and stably transforms a large fraction of infected canine D-17 cells. Using a colony-forming assay, we demonstrate stable transformation of 37% of cells that survive the infection. However, maximal transformation efficiency is achieved at doses of the E1-deleted recombinant adenoviruses that are toxic to the infected cells. Consequently, E1-deleted vector toxicity imposes a limitation on our current vector system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Adenoviridae
/
Herpesvirus 4, Human
/
Genetic Vectors
Limits:
Animals
/
Humans
Language:
En
Journal:
J Virol
Year:
1999
Document type:
Article
Affiliation country:
United States