Homologous serum-stimulated fibronectin synthesis in human retinal pigmented epithelial cells.
Aust N Z J Ophthalmol
; 27(3-4): 247-9, 1999.
Article
in En
| MEDLINE
| ID: mdl-10484205
ABSTRACT
PURPOSE:
Fibronectin expression has been recorded in subretinal membranes from patients with proliferative vitreoretinopathy (PVR). Retinal pigmented epithelial (RPE) cells are a major cellular component of PVR membranes and synthesize fibronectin. We investigated the effects of human serum (as a model of vascular leakage in vivo) on the expression of fibronectin by cultured human RPE cells and compared the responses to those stimulated by fetal bovine serum (FBS).METHODS:
Human RPE cells were incubated in M199 with 1, 10 and 40% human adult serum or FBS for 72 h.RESULTS:
Retinal pigmented epithelial cells expressed maximum extracellular matrix fibronectin when exposed to 40% human serum using immunohistochemistry. Using ELISA to quantify fibronectin, 10 and 40% human serum significantly increased the total fibronectin (P < 0.01; n = 4), whereas FBS did not affect fibronectin expression.CONCLUSIONS:
The results show that human serum contains substances stimulating fibronectin synthesis in human RPE cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pigment Epithelium of Eye
/
Blood Physiological Phenomena
/
Fibronectins
Limits:
Humans
Language:
En
Journal:
Aust N Z J Ophthalmol
Journal subject:
OFTALMOLOGIA
Year:
1999
Document type:
Article
Affiliation country:
Australia