In vivo activation and in situ BDNF-stimulated nuclear translocation of mitogen-activated/extracellular signal-regulated protein kinase is inhibited by ethanol in the developing rat hippocampus.
Neurosci Lett
; 272(2): 95-8, 1999 Sep 10.
Article
in En
| MEDLINE
| ID: mdl-10507550
In order to test the hypothesis that ethanol (EtOH)-induced changes in growth factor signal transduction contribute to the teratogenic effects of EtOH in the developing brain, neonatal rat pups were administered a single dose of EtOH during the brain growth spurt (5 days of age, PN5). Hippocampal mitogen-activated/extracellular signal-regulated protein kinase (MAPK/ERK) activation was analyzed one to 6 h after exposure by electrophoretic-mobility shift assay combined with western blot. Brain-Derived Neurotrophic Factor (BDNF) was used to stimulate ERK in hippocampal slices prepared from PN5 pups and activation and cellular localization was determined with immunofluorescence combined with confocal microscopy. EtOH decreased ERK activation in vivo and decreased nuclear translocation of BDNF-stimulated ERK in situ. These data suggest EtOH-induced inhibition of growth factor signaling may contribute to the development of fetal alcohol syndrome and alcohol-related birth defects.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain-Derived Neurotrophic Factor
/
Mitogen-Activated Protein Kinases
/
Ethanol
/
Hippocampus
Limits:
Animals
Language:
En
Journal:
Neurosci Lett
Year:
1999
Document type:
Article
Affiliation country:
United States
Country of publication:
Ireland