Endothelin-1 effect on tyrosine phosphorylation and on tyrosine phosphatase (PTP-1C) translocation in rabbit platelets.
J Recept Signal Transduct Res
; 19(6): 909-25, 1999 Nov.
Article
in En
| MEDLINE
| ID: mdl-10533980
ABSTRACT
This study examined the temporal relationships of endothelin-1-stimulated rabbit platelets tyrosine phosphorylated proteins. The effect of endothelin-1 on tyrosine phosphorylation was dose- and time-dependent and caused a rapid tyrosine phosphorylation of three groups of proteins in the molecular mass range 70-100 kDa, 100-150 kDa and 150-200 kDa. Significant protein tyrosine phosphatase activity and amount were found to be associated with the cytoskeleton of endothelin-1-stimulated rabbit platelets. Under our experimental conditions, translocation from the cytosolic fraction to the cytoskeleton reached its highest levels within 10-20 sec of endothelin-1 stimulation. Endothelin-1-induced translocation of protein tyrosine phosphatase, associated with the increase in its activity was demonstrated by immunoblotting and immunoelectron microscopy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Platelets
/
Signal Transduction
/
Protein Tyrosine Phosphatases
/
Endothelin-1
Limits:
Animals
Language:
En
Journal:
J Recept Signal Transduct Res
Journal subject:
BIOQUIMICA
/
FISIOLOGIA
Year:
1999
Document type:
Article
Affiliation country:
Spain