Genetic evidence for distinct roles of COX-1 and COX-2 in the immediate and delayed phases of prostaglandin synthesis in mast cells.
Biochem Biophys Res Commun
; 265(1): 205-10, 1999 Nov.
Article
in En
| MEDLINE
| ID: mdl-10548515
ABSTRACT
Activation of mast cells by aggregation of their high-affinity IgE receptors stimulates prostaglandin (PG) D(2) synthesis and secretion. An immediate phase of PGD(2) synthesis, complete within 30 min, is followed by a delayed, second phase of PGD(2) production that reaches a maximum 4 to 8 h after activation. Activation of mast cells from COX-2 (-/-) mice stimulates the release of PGD(2) during the first 30 min, whereas activation of mast cells from COX-1 (-/-) mice does not generate any PGD(2) in the first 2 h. On the other hand, COX-2 (-/-) cells do not participate in delayed phase of PGD(2) synthesis, while COX-1 (-/-) cells secrete low levels of PGD(2) between 2 and 4 h after activation. These data demonstrate that (i) the first phase of PG synthesis is COX-1 dependent and (ii) the second, delayed phase of PG synthesis is dependent on activation-induced synthesis and activity of COX-2.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prostaglandins
/
Prostaglandin-Endoperoxide Synthases
/
Isoenzymes
/
Mast Cells
Limits:
Animals
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
1999
Document type:
Article
Affiliation country:
United States