Hemocyanin subunit organization of the gastropod Rapana thomasiana.
Arch Biochem Biophys
; 372(1): 128-34, 1999 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-10562425
ABSTRACT
RtH1 and RtH2, the two hemocyanin isoforms of the prosobranch gastropod Rapana thomasiana, have been purified by anion-exchange chromatography and studied by SDS-PAGE and immunoelectrophoresis. Both subunit types are built up of eight functional units (FUs). Under reducing conditions subunit RtH2 splits into two fragments, RtH2-a-f and RtH2-gh, suggesting the presence of a disulfide bridge between FU2-f and FU2-g. By proteolytic cleavage of the subunits into three-, two-, and single-FU fragments, purification of fragments by HPLC, N-terminal sequencing of the peptides, and crossed-line immunoelectrophoresis, FUs-a-h of RtH2 and FU-a, FU-d, FU-e, and FU-f of RtH1 were identified and correlated to the eight-FUs pattern of immunoelectrophoresis. FU-a, FU-e, and FU-f of RtH1 and RtH2 are very closely related immunologically. RtH1 and RtH2 both correspond immunologically to KLH2, one of the two hemocyanin isoforms of the prosobranch gastropod Megathura crenulata.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hemocyanins
/
Mollusca
Limits:
Animals
Language:
En
Journal:
Arch Biochem Biophys
Year:
1999
Document type:
Article
Affiliation country:
Germany