Cyclin D3 regulates proliferation and apoptosis of leukemic T cell lines.
J Biol Chem
; 274(49): 34676-82, 1999 Dec 03.
Article
in En
| MEDLINE
| ID: mdl-10574933
ABSTRACT
Activation of the T cell receptor in leukemic T cell lines or T cell hybridomas causes growth inhibition. A similar growth inhibition is seen when protein kinase C is activated through addition of phorbol myristate acetate. This inhibition is due to an arrest of cell cycle progression in G(1) combined with an induction of apoptosis. Here we have investigated the mechanism by which these stimuli induce inhibition of proliferation in Jurkat and H9 leukemic T cell lines. We show that expression of cyclin D3 is reduced by each of these stimuli, resulting in a concomitant reduction in cyclin D-associated kinase activity. This reduction in cyclin D3-expression is crucial to the observed G(1) arrest, since ectopic expression of cyclin D3 can abrogate the G(1) arrest seen with each of these stimuli. Moreover, ectopic expression of cyclin D3 also prevents the induction of programmed cell death by phorbol myristate acetate and T-cell receptor activation, leading us to conclude that cyclin D3 not only plays a crucial role in progression through the G(1) phase, but is also involved in regulating apoptosis of T cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
Cyclins
Limits:
Humans
Language:
En
Journal:
J Biol Chem
Year:
1999
Document type:
Article
Affiliation country:
Netherlands