Induction of cytotoxic T lymphocyte activity by fusion-active peptide-containing virosomes.
Vaccine
; 18(14): 1327-33, 2000 Jan 31.
Article
in En
| MEDLINE
| ID: mdl-10618529
ABSTRACT
Priming of cytotoxic T lymphocyte (CTL) activity with exogenous antigen requires introduction of the antigen into the MHC class I presentation pathway of antigen-presenting cells. In the present study, we used fusogenic reconstituted envelopes (virosomes), derived from influenza virus, as a carrier system for delivery of a synthetic soluble peptide corresponding to a major murine CTL epitope of the influenza virus nucleoprotein (NP). Virosomes containing encapsulated NP-peptide efficiently sensitized target cells for recognition by influenza-specific CTLs generated through priming of mice with infectious virus. Intramuscular immunization of mice with peptide-containing virosomes induced a potent class I MHC-restricted CTL response against influenza-infected target cells. By contrast, an equal dose of NP-peptide encapsulated in fusion-inactivated virosomes did not induce CTL activity, indicating an essential role of the membrane fusion activity of the virosomes in the induction of the response. Likewise, NP-peptide encapsulated in liposomes, NP-peptide mixed with empty virosomes and NP-peptide in IFA failed to induce a CTL response. These results demonstrate that fusion-active virosomes represent a promising delivery system for induction of class I MHC-restricted CTL activity with non-replicating viral antigens.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Orthomyxoviridae
/
T-Lymphocytes, Cytotoxic
/
Viral Envelope Proteins
/
Nucleoproteins
Limits:
Animals
Language:
En
Journal:
Vaccine
Year:
2000
Document type:
Article
Affiliation country:
Netherlands
Country of publication:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS