Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages.
J Leukoc Biol
; 67(1): 104-8, 2000 Jan.
Article
in En
| MEDLINE
| ID: mdl-10648004
ABSTRACT
Macrosialin, the mouse homolog of human CD68, is a heavily glycosylated transmembrane protein found almost exclusively in macrophages. Its function remains uncertain. It has a high affinity for oxidized low-density lipoprotein (LDL) in ligand blots and antibodies against the human homolog, CD68, inhibit the binding of oxidized LDL to a human monocyte-derived cell line (THP-1). However, there is still controversy as to whether macrosialin, found predominantly in late endosomes, is expressed at all on the plasma membrane. The present studies, done in thioglycollate-elicited peritoneal macrophages, confirm that macrosialin is predominantly intracellular but show clearly that 10-15% of it is expressed on the cell surface. Exchange with intracellular pools occurs at an extremely high rate. The results are compatible with a surface function, including internalization of bound ligands or adhesion to surfaces.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antigens, Differentiation, Myelomonocytic
/
Antigens, CD
/
Macrophages, Peritoneal
Limits:
Animals
/
Humans
Language:
En
Journal:
J Leukoc Biol
Year:
2000
Document type:
Article
Affiliation country:
United States