Fibroblast growth factor-2 stimulates phospholipase Cbeta in adult cardiomyocytes.
Biochem Cell Biol
; 77(6): 569-75, 1999.
Article
in En
| MEDLINE
| ID: mdl-10668634
ABSTRACT
Although fibroblast growth factor-2 (FGF-2) plays an important role in cardioprotection and growth, little is known about the signals triggered by it in the adult heart. We therefore examined FGF-2-induced effects on phosphoinositide-specific phospholipase C (PI-PLC) isozymes, which produce second messengers linked to the inotropic and hypertrophic response of the myocardium. FGF-2, administered by retrograde perfusion to the isolated heart, induced an increase in inositol-1,4,5-trisphosphate levels in the cytosol, as well as an increase in total PI-PLC activity associated with sarcolemmal and cytosolic fractions. Furthermore FGF-2 induced a time-dependent elevation in cardiomyocyte membrane-associated PLC gamma1 and PLC beta1 activities, assayed in immunoprecipitated fractions, and moreover, increased the membrane levels of PLC beta1 and PLC beta3. Activation of PLC beta is suggestive of FGF-2-induced cross-talk between FGF-receptor tyrosine kinase and G-protein-coupled signaling in adult cardiomyocytes and underscores the importance of FGF-2 in cardiac physiology.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Type C Phospholipases
/
Fibroblast Growth Factor 2
/
Isoenzymes
/
Myocardium
Limits:
Animals
Language:
En
Journal:
Biochem Cell Biol
Journal subject:
BIOQUIMICA
Year:
1999
Document type:
Article
Affiliation country:
Canada