Assessment of cell culture and polymerase chain reaction procedures for the detection of polioviruses in wastewater.
Bull World Health Organ
; 77(12): 973-80, 1999.
Article
in En
| MEDLINE
| ID: mdl-10680244
ABSTRACT
WHO considers that environmental surveillance for wild-type polioviruses is potentially important for surveillance for acute flaccid paralysis as a means of confirming eradication of poliomyelitis. The present study investigated methods for detecting polioviruses in a variety of water environments in South Africa. Most polioviruses were isolated on L20B mouse cells, which, however, were not selective 16 reoviruses and 8 enteroviruses, apparently animal strains, were also isolated on these cells. Vaccine strains of polioviruses were isolated from surface waters during and shortly after two rounds of mass vaccination of children in an informal settlement where there was no sewerage. The results demonstrated the feasibility of poliovirus surveillance in such settlements. It was also evident that neither poliovirus vaccine strains nor other viruses were likely to interfere significantly with the detection of wild-type polioviruses. Optimal isolation of polioviruses was accomplished by parallel inoculation of L20B mouse cells and at least the PLC/PRF/5 human liver and buffalo green monkey (BGM) kidney cell lines. Analysis of cell cultures using the polymerase chain reaction revealed that 319 test samples contained at least 263 human enteroviruses that failed to produce a cytopathogenic effect. This type of analysis thus significantly increased the sensitivity of enterovirus detection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sewage
/
Water Microbiology
/
Environmental Monitoring
/
Polymerase Chain Reaction
/
Poliovirus
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Africa
Language:
En
Journal:
Bull World Health Organ
Year:
1999
Document type:
Article
Affiliation country:
South Africa
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND