Myosin light chain phosphorylation-dependent modulation of volume-regulated anion channels in macrovascular endothelium.
FEBS Lett
; 466(2-3): 346-50, 2000 Jan 28.
Article
in En
| MEDLINE
| ID: mdl-10682857
ABSTRACT
The Rho/Rho-associated kinase (ROK) pathway has been shown to modulate volume-regulated anion channels (VRAC) in cultured calf pulmonary artery endothelial (CPAE) cells. Since Rho/ROK can increase myosin light chain phosphorylation, we have now studied the effects of inhibitors of myosin light chain kinase (MLCK) or myosin light chain phosphatase (MLCP) on VRAC in CPAE. Application of ML-9, an MLCK inhibitor, inhibited VRAC, both when applied extracellularly or when dialyzed into the cell. A similar inhibitory effect was obtained by dialyzing the cells with AV25, a specific MLCK inhibitory peptide. Conversely, NIPP1(191-210), an MLCP inhibitory peptide, potentiated the activation of VRAC by a 25% hypotonic stimulus. These data indicate that activation of VRAC is modulated by MLC phosphorylation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endothelium, Vascular
/
Myosin Light Chains
/
Ion Channels
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
2000
Document type:
Article
Affiliation country:
Belgium