Cytotoxic and antitumor effect of fibrinogen-methotrexate conjugate.
Cancer Lett
; 148(2): 189-95, 2000 Feb 01.
Article
in En
| MEDLINE
| ID: mdl-10695996
ABSTRACT
In this paper we describe the chemical procedure of fibrinogen-methotrexate (F-MTX) conjugate preparation and its in vitro and in vivo antitumor activity. F-MTX conjugates were synthesized in reaction of fibrinogen with MTX N-hydroxysuccynimide ester. The conjugates were not cross-linked and were soluble in water. The results of the in vitro and in vivo studies have shown (1) a lower in vitro cytotoxicity of the F-MTX conjugate as compared with MTX alone; (2) a significantly higher in vivo antitumor activity of the F-MTX conjugate in mice with P388 leukemia as compared with MTX alone; (3) a significantly increased in vivo lethal toxicity of F-MTX as compared with MTX. The results suggest the therapeutic utility of the fibrinogen-methotrexate conjugate and the usefulness of fibrinogen as a chemotherapeutic drug carrier. However, a new effort in the preparation of F-MTX conjugate should be made to decrease its in vivo toxicity.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibrinogen
/
Methotrexate
/
Immunotoxins
/
Antineoplastic Agents
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Cancer Lett
Year:
2000
Document type:
Article
Affiliation country:
Poland