Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines.
Appl Environ Microbiol
; 66(3): 1216-9, 2000 Mar.
Article
in En
| MEDLINE
| ID: mdl-10698796
ABSTRACT
We report the novel application of a herbicide-resistance-based dominant marker for the positive selection of expression plasmids in Salmonella serovar vaccines. The beta-lactamase gene of the plasmid pTETnir15, which expresses fragment C of tetanus toxin (TetC), has been replaced with the bar gene marker. The new plasmid pBAT1 can be positively selected in vitro within Salmonella serovars in the presence of the herbicide DL-phosphinothricin. The expression of TetC remains unaltered, and the Salmonella enterica serovar Typhimurium vaccine strain is stable and immunogenic in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmids
/
Selection, Genetic
/
Acetyltransferases
/
Typhoid-Paratyphoid Vaccines
/
Genetic Markers
/
Aminobutyrates
Language:
En
Journal:
Appl Environ Microbiol
Year:
2000
Document type:
Article
Affiliation country:
United kingdom