Fungal endophthalmitis diagnosis by detection of Candida albicans DNA in intraocular fluid by use of a species-specific polymerase chain reaction assay.
J Infect Dis
; 181(3): 1198-201, 2000 Mar.
Article
in En
| MEDLINE
| ID: mdl-10720555
ABSTRACT
Candida endophthalmitis is a serious infection secondary to hematogenous dissemination or direct inoculation of the organisms following trauma or eye surgery. The diagnosis is based on the characteristic findings in the infected eye and on culture of vitreous samples. Unfortunately, the yield of vitreous cultures is limited. The use of a Candida albicans species-specific polymerase chain reaction (PCR) assay in the diagnosis of Candida endophthalmitis is reported herein. Four patients with suspected fungal endophthalmitis underwent vitrectomy for diagnostic and therapeutic purposes. In 2 of the 4, vitreous cultures were negative. However, characteristic PCR products were generated in all 4 patient specimens, enabling the rapid diagnosis of Candida endophthalmitis in all 4. Clinical response was observed in all cases. These results demonstrate the utility of PCR-mediated detection of C. albicans in vitreous samples.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Aqueous Humor
/
Candida albicans
/
Candidiasis
/
DNA, Fungal
/
Endophthalmitis
/
Polymerase Chain Reaction
Type of study:
Diagnostic_studies
Limits:
Adult
/
Aged
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Infect Dis
Year:
2000
Document type:
Article
Affiliation country:
United States