NF-kappaB inhibits apoptosis in murine mammary epithelia.
J Biol Chem
; 275(17): 12737-42, 2000 Apr 28.
Article
in En
| MEDLINE
| ID: mdl-10777569
ABSTRACT
The transcription factor NF-kappaB is a key modulator of apoptosis in a variety of cell types, but to date this specific function of NF-kappaB has not been demonstrated in epithelia. Here, we describe the activation of NF-kappaB during post-lactational involution of the mouse mammary gland, a period of extensive apoptosis of luminal epithelial cells. Significantly, active NF-kappaB localized exclusively to nonapoptotic epithelial cells both in vivo and in the mammary epithelial cell line, KIM-2, transduced with an NF-kappaB-dependent green fluorescent protein reporter. Activation of NF-kappaB in vitro coincided with a decrease in the cytosolic repressor, IkappaBalpha. Furthermore, induction of NF-kappaB either by extracellular ligands or, more specifically, by inhibition of the IkappaB repressor with adenoviral constructs expressing antisense mRNA, resulted in enhanced survival of KIM-2 cells. Therefore, although coincident with induction of apoptosis both in vivo and in vitro, NF-kappaB appeared to exert a selective survival function in epithelial cells. This study highlights for the first time a role for NF-kappaB in modulating apoptosis in epithelium.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
NF-kappa B
/
Apoptosis
/
Mammary Glands, Animal
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
J Biol Chem
Year:
2000
Document type:
Article
Affiliation country:
United kingdom