Heterogeneity of 11beta-hydroxysteroid dehydrogenase type 1/microsomal carbonyl reductase (11beta-HSD/CR) in guinea pig tissues. Purification of the liver form suggests modification in the cosubstrate binding site.
Toxicology
; 144(1-3): 63-9, 2000 Apr 03.
Article
in En
| MEDLINE
| ID: mdl-10781872
ABSTRACT
11beta-hydroxysteroid dehydrogenase (11beta-HSD) and xenobiotic carbonyl reductase activities were determined in guinea pig tissue microsomes. The data indicate the presence of a NADP(H) dependent form, distinct from the known type I isozyme. Purification of 11beta-HSD-1 from liver microsomes resulted in two distinct peaks, resolved by dye-ligand chromatography, indicating differences in the cosubstrate binding site. Immunoblot analysis using anti 11beta-HSD-1 antibodies reveals the presence of similar structural determinants between the enzyme forms. Both have an apparent molecular mass of 32 kDa, suggesting protein modifications occurring in the type 1 isozyme which account for the differences in chromatographic behaviour.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xenobiotics
/
Hydroxysteroid Dehydrogenases
/
Liver
Limits:
Animals
Language:
En
Journal:
Toxicology
Year:
2000
Document type:
Article
Affiliation country:
Sweden