Atomic structure of PDE4: insights into phosphodiesterase mechanism and specificity.
Science
; 288(5472): 1822-5, 2000 Jun 09.
Article
in En
| MEDLINE
| ID: mdl-10846163
ABSTRACT
Cyclic nucleotides are second messengers that are essential in vision, muscle contraction, neurotransmission, exocytosis, cell growth, and differentiation. These molecules are degraded by a family of enzymes known as phosphodiesterases, which serve a critical function by regulating the intracellular concentration of cyclic nucleotides. We have determined the three-dimensional structure of the catalytic domain of phosphodiesterase 4B2B to 1.77 angstrom resolution. The active site has been identified and contains a cluster of two metal atoms. The structure suggests the mechanism of action and basis for specificity and will provide a framework for structure-assisted drug design for members of the phosphodiesterase family.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cyclic AMP
/
3',5'-Cyclic-AMP Phosphodiesterases
Language:
En
Journal:
Science
Year:
2000
Document type:
Article
Affiliation country:
United States