Analysis of urinary nucleosides. I. Optimisation of high performance liquid chromatography/electrospray mass spectrometry.
Rapid Commun Mass Spectrom
; 14(14): 1200-7, 2000.
Article
in En
| MEDLINE
| ID: mdl-10918368
ABSTRACT
In order to optimise the analysis of urinary nucleosides by high performance liquid chromatography/mass spectrometry (HPLC/MS), the HPLC separation of these compounds was performed at different 'flow rates' and 0.2mL/min was found to give both a better separation and ionisation. The ionisation conditions were optimised to give the best intensity of the molecules quasi-molecular ions. The ion distribution profile and ionisation in both positive and negative mode were examined and the detection of the protonated molecule in positive mode chosen for further analysis. The limits of detection of the method developed are reported and representative LC/MS and LC/MS/MS spectra shown. Typical urinary nucleoside chromatograms are presented.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Chromatography, High Pressure Liquid
/
Nucleosides
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2000
Document type:
Article
Affiliation country:
United kingdom