Evaluation of LightCycler PCR for implementation of laboratory diagnosis of herpes simplex virus infections.
J Clin Microbiol
; 38(8): 3116-8, 2000 Aug.
Article
in En
| MEDLINE
| ID: mdl-10921993
ABSTRACT
Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive (n = 197; 23.1%) than cell cultures (n = 150) for the routine laboratory detection of herpes simplex virus infections.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Simplexvirus
/
Herpes Simplex
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2000
Document type:
Article
Affiliation country:
United States