Induction of the c-jun protooncogene expression by areca nut extract and arecoline on oral mucosal fibroblasts.
Oral Oncol
; 36(5): 432-6, 2000 Sep.
Article
in En
| MEDLINE
| ID: mdl-10964049
ABSTRACT
To investigate the mechanisms of areca quid (AQ)-induced carcinogenesis, expression of c-fos and c-jun protooncogenes was examined in human oral mucosal fibroblasts after exposure to areca nut extracts (ANE) or arecoline. We found that treatment of cells with 200 microg/ml ANE or 10 microg/ml arecoline for 1 h induced about three-fold increase in c-jun mRNA levels. This increase was transient and the level of c-jun mRNAs returned rapidly to control levels thereafter. However, ANE and arecoline did not induce c-fos mRNA expression at detectable levels. During AQ chewing, oral mucosal cells are continuously stimulated by ANE and arecoline. Persistent induction of the c-jun protooncogene by ANE and arecoline may be one of the mechanisms in the carcinogenesis of oral squamous cell carcinoma in Taiwan. Furthermore, we observed that pre-incubation of cells with either N-acetyl-cysteine [a glutathione (GSH) precursor] or L-buthionine-S,R-sulfoximine (a specific inhibitor of GSH biosynthesis) had a minimal effect on arecoline-induced c-jun expression. Therefore, arecoline-induced c-jun expression is independent of GSH depletion.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Areca
/
Arecoline
/
Plants, Medicinal
/
Mouth Neoplasms
/
Carcinoma, Squamous Cell
/
Proto-Oncogene Proteins c-jun
/
Fibroblasts
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Oral Oncol
Journal subject:
NEOPLASIAS
Year:
2000
Document type:
Article
Affiliation country:
Taiwan