Cloning, expression, and characterization of DNA polymerase I from the hyperthermophilic archaea Thermococcus fumicolans.

Cambon-Bonavita, M A; Schmitt, P; Zieger, M; Flaman, J M; Lesongeur, F; Raguénès, G; Bindel, D; Frisch, N; Lakkis, Z; Dupret, D; Barbier, G; Quérellou, J

Cambon-Bonavita, M A; Schmitt, P; Zieger, M; Flaman, J M; Lesongeur, F; Raguénès, G; Bindel, D; Frisch, N; Lakkis, Z; Dupret, D; Barbier, G; Quérellou, J.

Affiliation

Cambon-Bonavita MA; Laboratoire de Caractérisation des Microorganismes Marins, DRV/VP, Ifremer centre de Brest, Plouzané, France.

Extremophiles ; 4(4): 215-25, 2000 Aug.

Article in En | MEDLINE | ID: mdl-10972190

Subject(s)

DNA Polymerase I/genetics; Thermococcus/enzymology; Cloning, Molecular; DNA Polymerase I/isolation & purification; DNA Polymerase I/metabolism; Enzyme Stability; Escherichia coli; Exonucleases/genetics; Exonucleases/isolation & purification; Exonucleases/metabolism; Magnesium/pharmacology; Polymerase Chain Reaction; Protein Splicing; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Analysis, DNA; Temperature; Thermococcus/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Thermococcus / DNA Polymerase I Language: En Journal: Extremophiles Journal subject: BIOLOGIA Year: 2000 Document type: Article Affiliation country: France

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