Optical mapping of BAC clones from the human Y chromosome DAZ locus.
Genome Res
; 10(9): 1421-9, 2000 Sep.
Article
in En
| MEDLINE
| ID: mdl-10984460
ABSTRACT
The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Y Chromosome
/
Chromosome Mapping
/
Chromosomes, Bacterial
/
RNA-Binding Proteins
Limits:
Humans
Language:
En
Journal:
Genome Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2000
Document type:
Article
Affiliation country:
United States
Country of publication:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA