Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus.
Acta Crystallogr D Biol Crystallogr
; 56(Pt 10): 1278-87, 2000 Oct.
Article
in En
| MEDLINE
| ID: mdl-10998624
ABSTRACT
The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 A resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Conformation
/
Semliki forest virus
/
Image Processing, Computer-Assisted
/
Nucleocapsid
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta Crystallogr D Biol Crystallogr
Year:
2000
Document type:
Article
Affiliation country:
Germany
Country of publication:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA