Selective Rep-Cap gene amplification as a mechanism for high-titer recombinant AAV production from stable cell lines.

Liu, X; Voulgaropoulou, F; Chen, R; Johnson, P R; Clark, K R

Liu, X; Voulgaropoulou, F; Chen, R; Johnson, P R; Clark, K R.

Affiliation

Liu X; Children's Research Institute, Children's Hospital Inc., Ohio State University, Columbus, Ohio 43205, USA.

Mol Ther ; 2(4): 394-403, 2000 Oct.

Article in En | MEDLINE | ID: mdl-11020356

Subject(s)

DNA Replication/genetics; DNA, Viral/genetics; Dependovirus/genetics; Gene Amplification; Genes, Viral/genetics; Adenoviridae/genetics; Adenoviridae/metabolism; Blotting, Western; Cells, Cultured/cytology; Cells, Cultured/virology; DNA Primers/chemistry; DNA, Recombinant/genetics; DNA, Recombinant/metabolism; Dependovirus/metabolism; Fluorescent Antibody Technique; Gene Transfer Techniques; Genetic Vectors/administration & dosage; HeLa Cells/cytology; HeLa Cells/virology; Helper Viruses/genetics; Helper Viruses/metabolism; Humans; In Situ Hybridization; Plasmids/genetics; Plasmids/metabolism; Polymerase Chain Reaction; Transfection; Virus Replication; beta-Galactosidase/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: DNA, Viral / Gene Amplification / Dependovirus / DNA Replication / Genes, Viral Limits: Humans Language: En Journal: Mol Ther Journal subject: BIOLOGIA MOLECULAR / TERAPEUTICA Year: 2000 Document type: Article Affiliation country: United States Country of publication: United States

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