Influence of initial semen quality on the integrity of human sperm DNA following semen processing.
Fertil Steril
; 74(4): 824-7, 2000 Oct.
Article
in En
| MEDLINE
| ID: mdl-11020532
ABSTRACT
OBJECTIVE:
To examine and compare the effects of density-gradient centrifugation on the integrity of sperm DNA from the semen of both fertile and infertile men.DESIGN:
Prospective, observational study.SETTING:
University infertility clinic. PATIENTS Forty-four nonazoospermic, infertile men and nine fertile controls.INTERVENTIONS:
Semen samples were processed by density-gradient centrifugation. Sperm motility and sperm chromatin structure (evaluated by flow cytometry analysis of acridine orange-treated spermatozoa) were monitored before and after semen was processed. MAIN OUTCOMEMEASURES:
Sperm motility and DNA integrity.RESULTS:
Following density-gradient centrifugation, mean sperm motility (+/-SEM) improved significantly compared to whole semen in samples from fertile and infertile men, respectively (71 +/- 6 vs. 49 +/- 7% and 56 +/- 3 vs. 44 +/- 3%, P<0.05). However, the percentage of sperm with denatured DNA increased compared to whole semen after processing of samples from infertile (25 +/- 3 vs. 15 +/- 2%, P<0. 01) but not fertile men (9 +/- 3 vs. 8 +/- 2%, P>0.05).CONCLUSIONS:
Our data indicate that the potential detrimental effect of density-gradient centrifugation on sperm DNA integrity is related to the initial semen quality. These data urge us to examine our current sperm-processing techniques to minimize sperm DNA damage.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Semen
/
Semen Preservation
/
DNA
Type of study:
Observational_studies
Limits:
Adult
/
Humans
/
Male
Language:
En
Journal:
Fertil Steril
Year:
2000
Document type:
Article
Affiliation country:
Canada