Large-scale expression, refolding, and purification of the catalytic domain of human macrophage metalloelastase (MMP-12) in Escherichia coli.

Parkar, A A; Stow, M D; Smith, K; Panicker, A K; Guilloteau, J P; Jupp, R; Crowe, S J

Parkar, A A; Stow, M D; Smith, K; Panicker, A K; Guilloteau, J P; Jupp, R; Crowe, S J.

Affiliation

Parkar AA; Respiratory and RA Disease Group, Aventi Pharmaceuticals, Bridgewater, New Jersey 08807-0800, USA. Ash.Parkar@aventis.com

Protein Expr Purif ; 20(2): 152-61, 2000 Nov.

Article in En | MEDLINE | ID: mdl-11049739

Subject(s)

Catalytic Domain; Macrophages/enzymology; Metalloendopeptidases/chemistry; Metalloendopeptidases/isolation & purification; Protein Folding; Cloning, Molecular; Escherichia coli; Fluorometry; Humans; Inclusion Bodies; Light; Mass Spectrometry; Matrix Metalloproteinase 12; Metalloendopeptidases/genetics; Metalloendopeptidases/metabolism; Protein Denaturation/drug effects; Protein Renaturation; Protein Structure, Tertiary; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Scattering, Radiation; Solubility; Urea/pharmacology; alpha 1-Antitrypsin/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Metalloendopeptidases / Protein Folding / Catalytic Domain / Macrophages Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2000 Document type: Article Affiliation country: United States Country of publication: United States

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