Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B.
Acta Crystallogr D Biol Crystallogr
; 56(Pt 11): 1479-81, 2000 Nov.
Article
in En
| MEDLINE
| ID: mdl-11053859
ABSTRACT
A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2(1)2(1)2(1), with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) A and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 A resolution using synchrotron radiation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Cycle Proteins
Limits:
Humans
Language:
En
Journal:
Acta Crystallogr D Biol Crystallogr
Year:
2000
Document type:
Article
Affiliation country:
Japan