Platelet-activating factor-induced intracellular calcium waves in human uterine myometrial cells.
Eur J Obstet Gynecol Reprod Biol
; 93(2): 147-50, 2000 Dec.
Article
in En
| MEDLINE
| ID: mdl-11074135
ABSTRACT
OBJECTIVE:
We visualized and investigated the intracellular calcium waves propagated by platelet-activating factor (PAF) in cultured human myometrial cells. STUDYDESIGN:
Myometrial cells were stimulated with PAF ranging between 10(-8) and 10(-15) M. For the observation of calcium waves, calcium green-1 and a confocal laser microscopy were used. Cells were also stimulated with 10(-9) M of PAF in a calcium-free solution.RESULTS:
In physiological solution, PAF at concentrations ranging between 10(-9) and 10(-15) M induced intracellular calcium waves. Mean wave speed was 16.1+/-5.6 microm/s. Wave speeds were independent of the PAF concentration. Similar results were observed in the absence of added calcium, with the exception that the wave speeds were significantly slower (7.3+/-3.3 microm/s). CONCLUSIONS This is the first study to demonstrate the calcium waves propagated by PAF stimulation in human myometrial cells. These observations further support the proposed role of PAF in parturition.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Platelet Activating Factor
/
Calcium
/
Myometrium
Limits:
Female
/
Humans
Language:
En
Journal:
Eur J Obstet Gynecol Reprod Biol
Year:
2000
Document type:
Article
Affiliation country:
Japan