Fluorescent labelling of oligodeoxyribonucleotides by the oxyamino-aldehyde coupling reaction.

Trévisiol, E; Renard, A; Defrancq, E; Lhomme, J

Trévisiol, E; Renard, A; Defrancq, E; Lhomme, J.

Affiliation

Trévisiol E; Chimie BioOrganique, LEDSS, UMR 5616, Université Joseph Fourier, Grenoble, France.

Nucleosides Nucleotides Nucleic Acids ; 19(9): 1427-39, 2000 Sep.

Article in En | MEDLINE | ID: mdl-11092313

ABSTRACT

We describe the reaction of oligonucleotides containing an aldehydic group at the 5'-end or inside the sequence with an oxyamino label. The reaction was found to be highly selective and represents an efficient method for derivatization of oligonucleotides.

Subject(s)

Aldehydes/chemistry; Fluorescent Dyes/chemistry; Oligodeoxyribonucleotides/chemical synthesis; Chromatography, High Pressure Liquid; DNA/chemistry; Molecular Structure; Oligodeoxyribonucleotides/chemistry; Spectrometry, Fluorescence; Temperature

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Collection: 01-internacional Database: MEDLINE Main subject: Oligodeoxyribonucleotides / Aldehydes / Fluorescent Dyes Language: En Journal: Nucleosides Nucleotides Nucleic Acids Journal subject: BIOQUIMICA Year: 2000 Document type: Article Affiliation country: France Country of publication: United States

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