Molecular cloning and relative tissue expression of keratocan and mimecan in embryonic quail cornea.
Matrix Biol
; 19(7): 693-8, 2000 Dec.
Article
in En
| MEDLINE
| ID: mdl-11102758
ABSTRACT
We have cloned and sequenced the cDNAs for quail cornea keratan sulfate proteoglycan core proteins, keratocan and mimecan. The deduced quail keratocan protein contains a single conservative amino acid difference from the chick sequence, whereas quail mimecan protein contains a 58 amino acid-long avian-unique sequence that shares no homology with mammalian mimecan. Ribonuclease protection assay of Day 16 embryonic quail tissues reveals that keratocan and lumican are expressed at highest levels in cornea, whereas mimecan mRNA is expressed at a much lower level. Keratocan is expressed only in quail cornea, whereas mimecan is expressed in many different tissues as four transcripts of different sizes. Both lumican and mimecan are expressed at lowest levels in brain, liver and sternum.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteoglycans
/
Glycoproteins
/
Cornea
/
Eye Proteins
Limits:
Animals
Language:
En
Journal:
Matrix Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2000
Document type:
Article
Affiliation country:
United States
Country of publication:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS