Constitutive production of interleukin-1alpha mRNA and protein in the developing rat testis.
Int J Androl
; 23(6): 360-5, 2000 Dec.
Article
in En
| MEDLINE
| ID: mdl-11114982
ABSTRACT
Interleukin-1 (IL-1), a multifunctional cytokine produced mainly by activated macrophages, is also produced in the intact testis. Rat testicular IL-1 was found to be identical to IL-1alpha, judged by immunoneutralization of the bioactive protein and sequence comparison of cloned rat testicular and macrophage pro-IL-1alpha cDNA. Testicular IL-1alpha mRNA was first demonstrated on postnatal day 15, and the corresponding bioactive protein from day 20. IL-1alpha mRNA was still low on day 20, but then increased rapidly in parallel with the bioactive protein to establish a plateau level from day 25. In adult testes, IL-1alpha mRNA and immunoreactive protein were low in stage VII of the seminiferous epithelial cycle, whereas other stages showed a clearly detectable expression. In the adult testis, the concentration of IL-1alpha was 75 pg/mg testicular protein (approximately 200 pM). In conclusion, production of testicular IL-1alpha is developmentally and stage-dependently regulated, probably at the transcriptional level, emphasizing an important paracrine role in testicular function.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Testis
/
Interleukin-1
Limits:
Animals
Language:
En
Journal:
Int J Androl
Year:
2000
Document type:
Article
Affiliation country:
Sweden