Biological functions of trout pavement-like gill cells in primary culture on solid support: pH(i) regulation, cell volume regulation and xenobiotic biotransformation.
Comp Biochem Physiol A Mol Integr Physiol
; 128(2): 207-22, 2001 Feb.
Article
in En
| MEDLINE
| ID: mdl-11223382
ABSTRACT
This review presents results obtained on rainbow trout gill cells in primary culture on solid support. Ultrastructural analysis showed that cultured gill cells displayed features of pavement cells in situ. Several biological functions have been investigated on these cultured cells. First, it was shown that their intracellular pH at rest and after acidosis is regulated by a Na+/H+ exchanger. Second, gill cells in primary culture can regulate their volume after a cell swelling. Intracellular calcium appears to be involved in this regulation. The effects of different xenobiotics on the capacity of gill cells to regulate their volume are presented. Third, cultured pavement cells contain biotransformation enzymes to metabolize xenobiotics. All these results demonstrate that gill cells in primary culture on solid support represent a promising in vitro model for the study of pavement cells physiology. In conclusion, applications of this culture are discussed and compared with the permeable filter method, together with the limitations and prospects of this in vitro model on solid support.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xenobiotics
/
Oncorhynchus mykiss
/
Gills
/
Hydrogen-Ion Concentration
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol A Mol Integr Physiol
Journal subject:
BIOLOGIA MOLECULAR
/
FISIOLOGIA
Year:
2001
Document type:
Article
Affiliation country:
France