Induction of class II molecules by cytomegalovirus in rat heart endothelial cells is inhibited by ganciclovir.
Transpl Int
; 7 Suppl 1: S381-2, 1994.
Article
in En
| MEDLINE
| ID: mdl-11271258
ABSTRACT
Cytomegalovirus (CMV) has been demonstrated to induce class II antigen expression in endothelial cells. To study whether ganciclovir (DHPG) has an effect on CMV-induced class II expression, cultured rat heart endothelial cells were infected with rat CMV (RCMV) and treated with different DHPG concentrations. Class II antigens in endothelial cells were detected by a monoclonal antibody and immunoperoxidase technique. Control cells did not express class II antigen, but during RCMV infection 92% of cells were class II-positive. DHPG treatment (1, 10, 100 and 1000 microg/ml) decreased RCMV-induced class II expression from 73% to 59%, 6% and 0%, respectively. As DHPG inhibits CMV DNA polymerase, our present results suggest that DHPG affects RCMV-induced class II expression via the inhibition of RCMV DNA replication.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endothelium, Vascular
/
Histocompatibility Antigens Class II
/
Ganciclovir
/
Genes, MHC Class II
/
Cytomegalovirus
Limits:
Animals
Language:
En
Journal:
Transpl Int
Journal subject:
TRANSPLANTE
Year:
1994
Document type:
Article
Affiliation country:
Finland